PRF membranes: does angle of centrifuge matter?

That's a good question and I don't know the correct response. I'm not even sure if there has been any studies done on it. I've used a few different centrifuges. The BTI centrifuge for PRGF with the second button reprogrammed for the PRF spin and it worked pretty good. It has a horizontal rotor. The biggest difference I have seen is in the patient's themselves. There is just a lot of variation in blood from patient to patient.

The most important issue is the time you delay from take the sample and start centrifuge process..this variable will give a usable clot (with all the biomodifiers) and a not active one. The other thing is that you have about 2 hours to use the clot in your patient ,do not remove the tube from inside the centrifuge until you use it in the mouth. Dr. Hector Norero

Russell P. Fitton has said it best....... "The biggest difference I have seen is in the patient's themselves. There is just a lot of variation in blood from patient to patient." I have found in the more than 10 years we have been using PRP and PRF in our office, that the type of centrifuge does not make much of a difference......articles have been written about the speed and length of time of the spin, but I have not seen anything written on the angulation of the tubes in the machine. Using the same machines ( we have two) and the same technique to draw blood, there is a big variance in the end result, regardless of the age, sex, or size of the patient. It is very disappointing to obtain a red blob of mush, that falls apart when it is pressed to obtain a PRF membrane when the blood is drawn from a large healthy young man, and yet very decent, large yellow, fibrin clots can be harvested from a tiny 80 year od female; that will produce a large stretchable membrane . Gerald Rudick DDS Montreal, Canada

I doubt if the angle of the tubes is a big factor. An important part of the process is to get the drawn blood spinning as soon as possible so that the whole blood does not start to congeal. The best source for your answer would be to call Dr. Jim Rutkowski or Dr. Choukroun who are the experts in this technology.

I just switched over to a new system that is CGF- concentrated growth factors. It uses a special centrifuge that has a fixed 33 degree angle to it. The spin cycle is 13 minutes but at a variable rate. 2400rpm for the first couple of minutes then 2700rpm's for a few more minutes then finishes at 3000 rpm's. Supposedly this gives you a denser fibrinogen matrix and more growth factors that release slower into the wound site. Since I now have all , (PRGF,PRF,& CGF) I will draw 3 tubes on myself and let you know if I see any big difference in the fibrin clot I get. It makes the most sense to me that since there would be a great variance in the amount of platelet and thus growth factors from person to person that you would want as dense a fibrinogen clot as possible to give the patient a more sustained release of whatever growth factors you harvest from them.