PRF Protocol: What kind of vacutainer?

The L-PRF protocol requires the use of a red top vacutainer. This color indicates that there is no anti-coagulent present (sodium citrate). There are two types of tubes, however, that contain red tops; one is plastic and the other glass. The plastic tubes contain a silicate coating that initiates the clumping of the RBC's (rouleaux formation) which results in separation of the cell components. The glass tubes have natural silica so that a coating is not necessary for separation. RJM

Thanks for the clarification.

I use the 9 ml red top tubes with the soft top from BD.

We are using the new Advanced Platelet Rich Fibrin tubes from Dr. Choukroun. There are 10 ml glass tubes that comes in bags of 80. They are individually wrapped and sterilized with a traceability sticker. Venipuncture, PRF-Box and all the instruments are there as well as the centrifuge and TNT ships to North America with a minimal custom broker fee. All can be ordered individually, unlike in North America where the box used to be only for sale with the purchase of the centrifuge, very devious! Shipping is fast and relatively cheap, considering its coming from France.

Ayman, you'll receive soon the kit for A-PRF with the new tubes APRF10. During the free webinar you'll understand why I changed the composition of the tubes and the settings. And clinical and scientific outcolmes... see you soon.. Joseph

These "test tubes" also allow you to get PPP (Plasma Poor Protein) out of them. I Use the PPP at the end of surgery and the tissue heals faster. I love PRF and whenever I do lateral or crestal approach sinus lifts I use PRF. In tthe crestal, I try to pus two membranes in and then chop up one and mix it with the particulate boneand then tap to the length i am looking for. Robert miller turned me on to it. I actually have a service that takes the blood, spins it , and hands me the PRF,PPP. His only question to me is: " How many will you need. I use between 4-8 Tubes. I do not not want the delay of finding a vein and being frustrated before a complicated surgery. The only question

This might be a bit of a stretch, but can two or three good layers of PRF be used to cover the grafted site without the need for flap advancement? Tension free flap closer can be a bit demanding -after grafting- and might involve more tissue release than desired . Any comments will be appreciated.

I use the plugs all the time to graft sockets without a flap. Especially after reading recent journal; articles.The PRF-Box leaves plasma at the bottom for grafting.

It's possible to leave the PRF exposed. However, it depends on the biomaterial used..With the PRF the healing is excellent and the membrane is covered by progressive keratinization. With the A-PRF, it's completely different, the flaps are growing and after few days the site is completely closed. the release of BMP"s change completely the behavior of the healing.